Canine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity.
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Affiliation
Aston University, University of Chester, Veterinary Tissue Bank LtdPublication Date
2016-09-19
Metadata
Show full item recordAbstract
Mesenchymal stem cells (MSCs) have been used in cell replacement therapies for connective tissue damage, but also can stimulate wound healing through paracrine activity. In order to further understand the potential use of MSCs to treat dogs with neurological disorders, this study examined the paracrine action of adipose-derived canine MSCs on neuronal and endothelial cell models. The culture-expanded MSCs exhibited a MSC phenotype according to plastic adherence, cell morphology, CD profiling and differentiation potential along mesenchymal lineages. Treating the SH-SY5Y neuronal cell line with serum-free MSC culture-conditioned medium (MSC CM) significantly increased SH-SY5Y cell proliferation (P <0.01), neurite outgrowth (P = 0.0055) and immunopositivity for the neuronal marker βIII-tubulin (P = 0.0002). Treatment of the EA.hy926 endothelial cell line with MSC CM significantly increased the rate of wound closure in endothelial cell scratch wound assays (P = 0.0409), which was associated with significantly increased endothelial cell proliferation (P <0.05) and migration (P = 0.0001). Furthermore, canine MSC CM induced endothelial tubule formation in EA.hy926 cells in a soluble basement membrane matrix. Hence, this study has demonstrated that adipose-derived canine MSC CM stimulated neuronal and endothelial cells probably through the paracrine activity of MSC-secreted factors. This supports the use of canine MSC transplants or their secreted products in the clinical treatment of dogs with neurological disorders and provides some insight into possible mechanisms of action.Citation
Al Delfi, I. R., Sheard, J. J., Wood, C. R., Vernallis, A., Innes, J. F., Myint, P., & Johnson, W. E. B. (2016). Canine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity. The Veterinary Journal, 217, 10-17.Publisher
ElsevierJournal
The Veterinary JournalType
ArticleLanguage
enDescription
The lab-based study demonstrates that canine adipose-derived cells secrete factors that are angiogenic and neurogenic using cell assays. This activity has application in neural tissue repair.ae974a485f413a2113503eed53cd6c53
10.1016/j.tvjl.2016.09.003
Scopus Count
Collections
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/