Collections in this community

Recent Submissions

  • Combined bezafibrate, medroxyprogesterone acetate and valproic acid treatment inhibits osteosarcoma cell growth without adversely affecting normal mesenchymal stem cells

    Sheard, Jonathan J.; Southam, Andrew D.; MacKay, Hannah L.; Ellington, Max A.; Snow, Martyn D.; Khanim, Farhat L.; Bunce, Christopher M.; Johnson, William E.; orcid: 0000-0002-7247-9087 (Portland Press Ltd., 2021-01-05)
    Abstract Drug repurposing is a cost-effective means of targeting new therapies for cancer. We have examined the effects of the repurposed drugs, bezafibrate, medroxyprogesterone acetate and valproic acid on human osteosarcoma cells, i.e., SAOS2 and MG63 compared with their normal cell counterparts, i.e. mesenchymal stem/stromal cells (MSCs). Cell growth, viability and migration were measured by biochemical assay and live cell imaging, whilst levels of lipid-synthesising enzymes were measured by immunoblotting cell extracts. These drug treatments inhibited the growth and survival of SAOS2 and MG63 cells most effectively when used in combination (termed V-BAP). In contrast, V-BAP treated MSCs remained viable with only moderately reduced cell proliferation. V-BAP treatment also inhibited migratory cell phenotypes. MG63 and SAOS2 cells expressed much greater levels of fatty acid synthase and stearoyl CoA desaturase 1 than MSCs, but these elevated enzyme levels significantly decreased in the V-BAP treated osteosarcoma cells prior to cell death. Hence, we have identified a repurposed drug combination that selectively inhibits the growth and survival of human osteosarcoma cells in association with altered lipid metabolism without adversely affecting their non-transformed cell counterparts.
  • Burden of illness of progressive familial intrahepatic cholestasis in the US, UK, France, and Germany: study rationale and protocol of the PICTURE study

    Ruiz-Casas, Leonardo; O’Hara, Sonia; orcid: 0000-0002-9119-8336; Mighiu, Claudia; Finnegan, Alan; Taylor, Alison; Ventura, Emily; Dhawan, Anil; Murray, Karen F; Schattenberg, Jorn; orcid: 0000-0002-4224-4703; Willemse, Jose; et al. (Informa UK Limited, 2021-01-07)
  • Real-world evidence on Kovaltry (81-8973) in children with moderate or severe hemophilia A in Europe: a nested cohort analysis

    O’Hara, Jamie; Hirst, Ceri; orcid: 0000-0001-9094-2437; email: ceri.hirst@bayer.com; Cabre Marquez, Jose Francisco; Burke, Tom (BioMed Central, 2021-01-15)
    Abstract: Background: Untreated hemophilia A patients may experience recurrent bleeding events leading to debilitating joint damages. While RCT and pharmacokinetic data support the value of Kovaltry [an unmodified full-length recombinant factor VIII (FVIII) product], real world evidence in children is lacking. This report describes a descriptive and multivariate analysis of the effectiveness of Kovaltry in children with hemophilia A in the real-world setting, using data from medical chart abstraction and cross-sectional surveys of physicians, patients, and caregivers. Results: Male patients aged < 18 years with moderate or severe hemophilia A, residing in five European countries and treated with FVIII were studied. The co-primary endpoints were the annualized bleeding rate (ABR) and the annual FVIII utilization rate. Twenty nine patients treated with Kovaltry were included, of whom 93% had severe disease and 75% were on continuous prophylactic treatment. The mean ABR was 2.66 ± 2.06, with rates decreasing with age. The children received on average 2.45 infusions per week, consistent across age groups (median 3; range 1–3). There were no reports of inhibitor development or adverse events in the study (AEs), and all patients were satisfied or very satisfied with the treatment. An exploratory multivariate analysis suggests no significant difference in ABR or units utilized between Kovaltry and some extended half life products in children with severe hemophilia A, though characteristics of these patient cohorts were markedly different. Conclusion: This analysis demonstrates the effectiveness and safety of Kovaltry in a pan-European pediatric population with severe hemophilia A.
  • COVID-19 presenting as intussusception in infants: A case report with literature review

    Athamnah, Mohammad N.; Masade, Salim; Hamdallah, Hanady; orcid: 0000-0001-6314-0236; Banikhaled, Nasser; Shatnawi, Wafa; Elmughrabi, Marwa; Al Azzam, Hussein S.O. (Elsevier, 2021-01-12)
    The novel Corona virus disease 2019 (COVID-19) first presented in Wuhan, China. The virus was able to spread throughout the world, causing a global health crisis. The virus spread widely in Jordan after a wedding party held in northern Jordan. In most cases of COVID-19 infection, respiratory symptoms are predominant. However, in rare cases the disease may present with non-respiratory symptoms. The presentation of COVID-19 as a case of intussusception in children is a strange and rare phenomenon. We present here a case of a two-and-a-half month old male baby who was brought to hospital due to fever, frequent vomiting, dehydration and blood in stool. He was diagnosed as intussusception. The child was tested for corona due to the large societal spread of the virus and because he was near his mother, who was suffering from symptoms similar to corona or seasonal flu (she did not conduct a corona test). Patient was treated without surgery and recovered quickly. The COVID-19 infection was without respiratory symptoms, and there was no need for the child to remain in hospital after treatment of intussusception. The relationship between viruses, mesenteric lymphoid hyperplasia, and intussusception is a confirmed relation. ACE2 is the key receptor required for SARA-COV-2 to enter the host cells. ACE2 has been also found in the brush border of the intestinal mucosa, as well as it is a key inflammatory regulator in the intestine. This may suggest that SARSA-COV-2 could invade the respiratory tract as well as gastrointestinal tract or both. Few case reports documented the presentation of COVID-19 as intussusception in children. In the light of the wide-spread of corona virus, performing COVID-19 tests for children with intussusception can help linking the two entities. Development of gastrointestinal symptoms in COVID-19 positive children should raise concern about the development of intussusception.
  • Are Prisoners More Psychopathic than Non-forensic Populations? Profiling Psychopathic Traits among Prisoners, Community Adults, University Students, and Adolescents

    Boduszek, Daniel; orcid: 0000-0001-5863-2906; Debowska, Agata; Sherretts, Nicole; Willmott, Dominic; Boulton, Mike; Kielkiewicz, Krzysztof; Popiolek, Katarzyna; Hyland, Philip (Informa UK Limited, 2019-09-12)
  • Refining the blank line‐up procedure: How should we instruct eyewitnesses?

    Kucina, Talira; Sauer, James D.; Holt, Glenys A.; Brewer, Neil; Palmer, Matthew A.; orcid: 0000-0002-3467-3364 (Wiley, 2020-08-19)
  • A Review of Piezoelectric and Magnetostrictive Biosensor Materials for Detection of COVID‐19 and Other Viruses

    Narita, Fumio; orcid: 0000-0002-0957-1948; Wang, Zhenjin; orcid: 0000-0003-2546-6029; Kurita, Hiroki; Li, Zhen; Shi, Yu; Jia, Yu; Soutis, Constantinos (Wiley, 2020-11-24)
  • Effect of combined home-based, overground robotic-assisted gait training and usual physiotherapy on clinical functional outcomes in people with chronic stroke: A randomized controlled trial.

    Wright, Amy; orcid: 0000-0002-7006-6465; Stone, Keeron; Martinelli, Louis; Fryer, Simon; Smith, Grace; Lambrick, Danielle; Stoner, Lee; orcid: 0000-0002-0682-2270; Jobson, Simon; Faulkner, James; orcid: 0000-0002-3704-6737 (2020-12-27)
    To assess the effect of a home-based over-ground robotic-assisted gait training program using the AlterG Bionic Leg orthosis on clinical functional outcomes in people with chronic stroke. Randomized controlled trial. Home. Thirty-four ambulatory chronic stroke patients who recieve usual physiotherapy. Usual physiotherapy plus either (1)10-week over-ground robotic-assisted gait training program (  = 16), using the device for ⩾30 minutes per day, or (2) control group (  = 18), 30 minutes of physical activity per day. The primary outcome was the Six-Minute Walk Test. Secondary outcomes included: Timed-Up-and-Go, Functional Ambulation Categories, Dynamic Gait Index and Berg Balance Scale. Physical activity and sedentary time were assessed using accelerometry. All measurements were completed at baseline, 10 and 22 weeks after baseline. Significant increases in walking distance were observed for the Six-Minute Walk Test between baseline and 10 weeks for over-ground robotic-assisted gait training (135 ± 81 m vs 158 ± 93 m, respectively;  ⩽ 0.001) but not for control (122 ± 92 m vs 119 ± 84 m, respectively). Findings were similar for Functional Ambulation Categories, Dynamic Gait Index and Berg Balance Scale (all  ⩽ 0.01). For over-ground robotic-assisted gait training, there were increases in time spent stepping, number of steps taken, number of sit-to-stand transitions, and reductions in time spent sitting/supine between baseline and 10 weeks (all  < 0.05). The differences observed in all of the aforementioned outcome measures were maintained at 22 weeks, 12 weeks after completing the intervention (all  > 0.05). Over-ground robotic-assisted gait training combined with physiotherapy in chronic stroke patients led to significant improvements in clinical functional outcomes and physical activity compared to the control group. Improvements were maintained at 22 weeks.
  • Resilience and mindfulness in nurse training on an undergraduate curriculum.

    Mitchell, Andrew E P; orcid: 0000-0001-6244-5249 (2020-12-23)
    The aim is to investigate what relationships exist between resilience and mindfulness in undergraduate nurse training and how these might contribute to well-being. One hundred and six students participated in this cross-sectional study. Multivariate and bivariate procedures were utilized to assess the differences between students' demographics, academic resilience, and mindfulness. The findings suggested that acceptance and attention within mindfulness were important for resilience. Students who had higher levels of academic resilience also had higher indexes of mindfulness. A key implication is that learning and practice areas should ensure that well-being, mindfulness, and resilience literacy are key issues for students in training. This is at a time when mental health support and staff retention are foremost in policymakers' minds. [Abstract copyright: © 2020 Wiley Periodicals LLC.]
  • COVID-19 presenting as intussusception in infants: A case report with literature review

    Athamnah, Mohammad N.; Masade, Salim; Hamdallah, Hanady; orcid: 0000-0001-6314-0236; Banikhaled, Nasser; Shatnawi, Wafa; Elmughrabi, Marwa; Al Azzam, Hussein S.O.
    The novel Corona virus disease 2019 (COVID-19) first presented in Wuhan, China. The virus was able to spread throughout the world, causing a global health crisis. The virus spread widely in Jordan after a wedding party held in northern Jordan. In most cases of COVID-19 infection, respiratory symptoms are predominant. However, in rare cases the disease may present with non-respiratory symptoms. The presentation of COVID-19 as a case of intussusception in children is a strange and rare phenomenon. We present here a case of a two-and-a-half month old male baby who was brought to hospital due to fever, frequent vomiting, dehydration and blood in stool. He was diagnosed as intussusception. The child was tested for corona due to the large societal spread of the virus and because he was near his mother, who was suffering from symptoms similar to corona or seasonal flu (she did not conduct a corona test). Patient was treated without surgery and recovered quickly. The COVID-19 infection was without respiratory symptoms, and there was no need for the child to remain in hospital after treatment of intussusception. The relationship between viruses, mesenteric lymphoid hyperplasia, and intussusception is a confirmed relation. ACE2 is the key receptor required for SARA-COV-2 to enter the host cells. ACE2 has been also found in the brush border of the intestinal mucosa, as well as it is a key inflammatory regulator in the intestine. This may suggest that SARSA-COV-2 could invade the respiratory tract as well as gastrointestinal tract or both. Few case reports documented the presentation of COVID-19 as intussusception in children. In the light of the wide-spread of corona virus, performing COVID-19 tests for children with intussusception can help linking the two entities. Development of gastrointestinal symptoms in COVID-19 positive children should raise concern about the development of intussusception.
  • Double bordered constructions of self-dual codes from group rings over Frobenius rings

    Gildea, Joe; Taylor, Rhian; orcid: 0000-0002-8563-2212; email: rhian.taylor@chester.ac.uk; Kaya, Abidin; Tylyshchak, A. (Springer US, 2020-01-09)
    Abstract: In this work, we describe a double bordered construction of self-dual codes from group rings. We show that this construction is effective for groups of order 2p where p is odd, over the rings F2+uF2 and F4+uF4. We demonstrate the importance of this new construction by finding many new binary self-dual codes of lengths 64, 68 and 80; the new codes and their corresponding weight enumerators are listed in several tables.
  • Facilitating literature searches for work based learning students using an action research approach

    Talbot, Jon; orcid: 0000-0003-1234-5180; Bennett, Lee (Informa UK Limited, 2020-12-29)
  • Understanding minimum and ideal factor levels for participation in physical activities by people with haemophilia: An expert elicitation exercise

    Martin, Antony P.; orcid: 0000-0003-4383-6038; Burke, Tom; Asghar, Sohaib; Noone, Declan; Pedra, Gabriel; orcid: 0000-0002-2023-5224; O'Hara, Jamie (Wiley, 2020-04-08)
  • Effect of combined home-based, overground robotic-assisted gait training and usual physiotherapy on clinical functional outcomes in people with chronic stroke: A randomized controlled trial

    Wright, Amy; orcid: 0000-0002-7006-6465; Stone, Keeron; Martinelli, Louis; Fryer, Simon; Smith, Grace; Lambrick, Danielle; Stoner, Lee; orcid: 0000-0002-0682-2270; Jobson, Simon; Faulkner, James; orcid: 0000-0002-3704-6737 (SAGE Publications, 2020-12-27)
    Objectives: To assess the effect of a home-based over-ground robotic-assisted gait training program using the AlterG Bionic Leg orthosis on clinical functional outcomes in people with chronic stroke. Design: Randomized controlled trial. Setting: Home. Participants: Thirty-four ambulatory chronic stroke patients who recieve usual physiotherapy. Intervention: Usual physiotherapy plus either (1)10-week over-ground robotic-assisted gait training program ( n = 16), using the device for ⩾30 minutes per day, or (2) control group ( n = 18), 30 minutes of physical activity per day. Measurements: The primary outcome was the Six-Minute Walk Test. Secondary outcomes included: Timed-Up-and-Go, Functional Ambulation Categories, Dynamic Gait Index and Berg Balance Scale. Physical activity and sedentary time were assessed using accelerometry. All measurements were completed at baseline, 10 and 22 weeks after baseline. Results: Significant increases in walking distance were observed for the Six-Minute Walk Test between baseline and 10 weeks for over-ground robotic-assisted gait training (135 ± 81 m vs 158 ± 93 m, respectively; P ⩽ 0.001) but not for control (122 ± 92 m vs 119 ± 84 m, respectively). Findings were similar for Functional Ambulation Categories, Dynamic Gait Index and Berg Balance Scale (all P ⩽ 0.01). For over-ground robotic-assisted gait training, there were increases in time spent stepping, number of steps taken, number of sit-to-stand transitions, and reductions in time spent sitting/supine between baseline and 10 weeks (all P &lt; 0.05). The differences observed in all of the aforementioned outcome measures were maintained at 22 weeks, 12 weeks after completing the intervention (all P &gt; 0.05). Conclusion: Over-ground robotic-assisted gait training combined with physiotherapy in chronic stroke patients led to significant improvements in clinical functional outcomes and physical activity compared to the control group. Improvements were maintained at 22 weeks.
  • Resilience and mindfulness in nurse training on an undergraduate curriculum

    Mitchell, Andrew E. P.; orcid: 0000-0001-6244-5249 (Wiley, 2020-12-23)
  • Using historical source data to understand urban flood risk: a socio-hydrological modelling application at Gregório Creek, Brazil

    Sarmento Buarque, Ana Carolina; orcid: 0000-0002-9489-4214; Bhattacharya-Mis, Namrata; orcid: 0000-0003-4967-8325; Fava, Maria Clara; orcid: 0000-0002-8201-4339; Souza, Felipe Augusto Arguello de; orcid: 0000-0002-2753-9896; Mendiondo, Eduardo Mario; orcid: 0000-0003-2319-2773 (Informa UK Limited, 2020-04-01)
  • Pioneering British women chemists: their lives and contributions

    Badrock, Gill (Informa UK Limited, 2020-05-31)
  • “It is not the same”: relationships and dementia

    Benbow, Susan Mary; Tsaroucha, Anna; Sharman, Victoria (Informa UK Limited, 2019-08-28)

View more